Can living microorganisms be formed from non-living materials in broth by spontaneous generation?
Quantity; Materials:
1: Hot Plate
6: 50 ML Flask
2: Stopper (no holes)
2: Stopper (with holes)
2.4 grams: Nutrient Broth
300 mL : Water
1: 500 mL Autoclave bottle
1: 100 mL Graduated Cylinder
Procedure:
1. Clean all instruments, containers and materials with soap and water. After washing, flip flasks downward to reduce the amount of air exposure.
2. In autoclave glass bottle, create nutrient broth by adding 2.4 grams with 300 mL of water. Mix the solution by shaking the bottle with the cap on. :)
3. Divide the nutrient broth upon 6 flasks each containing .50 mL. 2 flasks will have no stoppers, 2 with stoppers that has holes and 2 stoppers with no holes. Use the funnel to distribute the broth to all flasks.
4. Separate flasks into 2 groups, Group 1 will all be boiled on the hot plate while Group 2 will all be in room temperature. Each group will have a flask with no stoppers, stoppers w/holes and stoppers w/ no holes.
5. By using an oven mitt, boil all flask in Group 1. Seal all flasks with stoppers!
6. Allow flasks to sit for 24 hours and observe the results! :D OBSERVE IT. LOOK AT IT.
Quantity; Materials:
1: Hot Plate
6: 50 ML Flask
2: Stopper (no holes)
2: Stopper (with holes)
2.4 grams: Nutrient Broth
300 mL : Water
1: 500 mL Autoclave bottle
1: 100 mL Graduated Cylinder
Procedure:
1. Clean all instruments, containers and materials with soap and water. After washing, flip flasks downward to reduce the amount of air exposure.
2. In autoclave glass bottle, create nutrient broth by adding 2.4 grams with 300 mL of water. Mix the solution by shaking the bottle with the cap on. :)
3. Divide the nutrient broth upon 6 flasks each containing .50 mL. 2 flasks will have no stoppers, 2 with stoppers that has holes and 2 stoppers with no holes. Use the funnel to distribute the broth to all flasks.
4. Separate flasks into 2 groups, Group 1 will all be boiled on the hot plate while Group 2 will all be in room temperature. Each group will have a flask with no stoppers, stoppers w/holes and stoppers w/ no holes.
5. By using an oven mitt, boil all flask in Group 1. Seal all flasks with stoppers!
6. Allow flasks to sit for 24 hours and observe the results! :D OBSERVE IT. LOOK AT IT.
Young lady creating the nutrient broth: 300 mL of water and 2.4 grams of nutrient broth powder.
IS IT STINKY???
A young lad dividing the nutrient broth upon 6 flasks, using a funnel and a graduated cylinder to measure out .50 mL
After separating the flasks into the 2 groups (flask with no stoppers, stoppers w/holes and stoppers w/ no holes in each group) Get ready to boil all flasks in group 1.
Make sure to wear oven mitts!!! LIKE THIS CLASSY FELLOW OVER THERE -->
BOIL THESE BABIES UP on your hot plate
Once the flasks in group 1 got to boiling point, seal them tight if they require a stopper. All flasks in group 2 that needs a stopper should be sealed before hand because they do not need to be boiled.
Let the flasks chill out for 24 hours
Don't forget to observe results and record your data :D
ENDING RESULTS:
The re-creation of experiments conducted by John Needham, Lazzaro Spallanzani and Louis Pasteur who proved and disproved biogenesis and spontaneous generation.
- Not boiled with stopper (control) had no exposure to air showed microbial growth. Cause of the growth was from human error, contamination from dirty flask or slight exposure to air from failing to close the flask with stopper quickly or spontaneous generation.
- Not boiled with stopper (w/ holes) limited exposure to air. Source of contamination was from exposure to microbes in the air. Biogenesis: microbes in the air lead to the growth of microorganisms in flask.
- Not boiled with no stopper, full exposure to air. Source of contamination was from exposure to microbes in the air. Biogenesis: microbes in the air lead to the growth of microorganisms in flask. This flask had the most growth, the nutrient broth was opaque and cloudy.
- Boiled with stopper (no holes) No exposure to air. Biogenesis: nothing grew in flask because there was no exposure to the microbes in the air. The nutrient broth stayed yellow and clear.
- Boiled with stopper (with holes) limited exposure to air. Source of contamination was from exposure to microbes in the air. Biogenesis: microbes in the air lead to the growth of microorganisms in flask.
- Boiled no stopper, full exposure to air. Source of contamination was from exposure to microbes in the air. Biogenesis: microbes in the air lead to the growth of microorganisms in flask.
The re-creation of experiments conducted by John Needham, Lazzaro Spallanzani and Louis Pasteur who proved and disproved biogenesis and spontaneous generation.